In Gastric Cancer, PCR-SSCP Is Being Performed to Recognize P53 Gene Mutations

Abstract

Gastric adenocarcinoma is the second most common cancer in the world after lung cancer. About 500,755 cases per year new is identified. Approximately 12% of all cancer deaths are due to gastric cancer. One of the most important the genes that are important in preventing cancer are the P53 gene, which is involved in regulating the cell cycle. In some of Up to 50% of cancers are mutated in the P53 gene, of which about 87% of mutations are in exons 5 to 8. are. This study was performed to determine p53 gene mutations in gastric cancer by SSCP-PCR. Materials and Methods: During 2002, 44 biopsy specimens of gastric cancer patients were prepared from three hospital centers in Tehran. Demographic characteristics of patients and samples of patients by SSCP-PCR method to determine mutations in P53 gene was studied. Results, in this study, out of 44 patients with gastric cancer, 31 were male and 13 were female with a mean age of 60.8 years (from 34 years). Up to 84 years). Based on the type of cancer, 36 cases were intestinal (81.8%) and 5 (11.4%) were diffuse. Three cases were not included in either of the two groups with the information available. After performing SSCP-PCR in 9 cases Electrophoretic bands of patients' samples were different from normal samples. One mutation in exon 5 (11.1%), 2 mutations in Exons (22.2%), 3 mutations in exon (33.3%) and 3 mutations in exon (33.3%) were observed. Of these 9 mutations, 7 were related to intestinal cancer and 2 were diffuse. Significant relationship between age and type of cancer Not observed. There is also a significant relationship between the type of cancer and sex, between sex and the location of the mutation, as well as the type of cancer the mutation site was not seen. In the diffuse type, 2 mutations were observed in exons 6 and 8 and no mutations were observed in exons 5 and 7. was not observed. Mutations were seen in the intestinal type in all four exons studied Conclusion in this study, the incidence of mutation in P53 gene in gastric cancer was 20.5% which indicates the frequency he means mutation of p53 gene is in the study population.